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We create a simple, rapid, equipment-free papertronic sensor array that is connected to a visual readout, allowing the naked eye to access antibiotic effectiveness against pathogenic bacteria, Pseudomonas aeruginosa PA01. The sensing approach quickly monitors microbial bioelectricity which is based on their metabolic activities and is inversely proportional to the concentration of antibiotics. Each sensing system consists of a two-electrode microbial sensing unit, an interface circuit for sensor signal amplification, and an electronic visual display with a light-emitting diode (LED), which are all mounted onto a paper-based printed circuit board. The bioelectricity in the sensing unit is amplified by the transistor and is transduced into LED illumination when a pre-defined electric current corresponding to a bacterial sample with a certain antibiotic concentration is obtained. Only within an hour, the system generates reliable, discrete visual responses to monitor antibiotic efficacy and provides the right doses for treatment against bacterial infections.